Lumicyte™ Blood Counting Chamber

Description

Lumicyte™ Blood Counting Chamber (Hemacytometer) provides absolute measuring and counting accuracy in a double-chamber format and counting grids developed by Neubauer. The chamber is sealed and provides high magnification for counting certainty.

• Bright-line metalized counting chamber.
• Designed for high visibility and comfort, especially under low-contrast conditions
• Features improved double Neubauer ruling
• Provides a high degree of accuracy and precision
• Precisely ground for even surface

Propper Hemacytometer Cover Glass is a ready-to-use companion with the modern-day hemacytometer double-chamber format of the Propper Blood Counting Chamber.

The Significance of Cell Counting in Various Biological Applications

Cell counting is an essential technique in fields such as microbiology, cell culture, and blood analysis. It is crucial for ensuring the success and accuracy of diverse biological experiments.

Cell Counting with Hemacytometer: A Historical Perspective

French anatomist Louis-Charles Malassez pioneered the technique of cell counting using a hemacytometer in the 19th century. Initially devised for blood cell counts, this method gained prominence due to its accuracy in conditions where automated counters falter, like abnormal blood cell counts.

Versatile Applications of Hemacytometer Cell Counting

Beyond blood cell counts, hemacytometers have various applications, including sperm counts, cell culture assessments tracking cell growth, phytoplankton cell quantification, and more. The hemacytometer’s significance extends to measuring cell size through micrographs, leveraging its known slide dimensions for precise estimations.

Utilizing the Hemacytometer Technique

While the underlying science of cell counting is intricate, using a hemacytometer is relatively straightforward. The device typically features an “H” shape encompassing two polished grid surfaces within the central chamber, which provides a cover slip area.

Once prepared, a sample-filled pipette tip is placed into a v-shaped well of the hemacytometer, and the sample is gently spread over the area. Adding about 10 µl of a specialized liquid under the cover slip ensures proper coverage. This technique allows for loading two samples concurrently, each using one of the two grids.

Subsequently, place the prepared hemacytometer on a microscope stage to visualize the counting grid at low magnification. After allowing the sample to settle, the counting process commences. To prevent duplication, tally all cells within the lines of the large square on the hemacytometer.

To achieve the desired cell count, we may dilute the liquid with different substances. For example, we can use specific liquid stains to differentiate between dead and live cells.

In conclusion, cell counting through the hemacytometer technique is a fundamental practice in diverse biological applications.

The Hemacytometer Cover Glass and Blood Counting Chamber has been an essential tool for medical practitioners and biologists.

“Helping hematologists conquer blood diseases worldwide.” — American Society of Hematology